Clematis terniflora DC.提取物抗炎、镇痛和免疫调节活性的体内和体外评价,以及乙酸橙酰胺的参与

An in vivo and in vitro assessment of the anti-inflammatory, antinociceptive, and immunomodulatory activities of Clematis terniflora DC. extract, participation of aurantiamide acetate
2015-08-11 09:12点击:385次发表评论
作者:Liu, X.B. , Yang, B.X., Zhang, L., Lu, Y.Z., Gong, M.H., Tian, J.K.
机构: 浙江大学生物医学工程和仪器科学学院
期刊: J ENDOUROL2015年7月期169卷

Abstract Aim Clematis terniflora DC. has been widely used as a traditional Chinese medicine for the treatment of tonsillitis, rheumatoid arthritis, and prostatitis. Despite its widespread use in China, there are currently no studies systematically examined its therapeutic effects and mechanism of action. As such, the present study was conducted to evaluate the anti-inflammatory, antinociceptive, and immunomodulatory effects of C. terniflora DC. using rodent and cellular models. Methods The anti-inflammatory properties of the 70% ethanol eluted fraction of the 70% ethanol extract of C. terniflora DC. (EECTD) were evaluated using the xylene-induced ear swelling test, the carrageenan-induced edema model, and the cotton pellet granuloma method. Its antinociceptive activities were determined using both the acetic acid-induced writhing test and the hot plate assay. In parallel, we conducted an in vitro assay in LPS-induced RAW264.7 cells to examine the anti-inflammatory effects of EECTD and its purified form, aurantiamide acetate (AA) on inhibition of nitric oxide (NO) and prostaglandin E2 (PGE2) release. Results EECTD (300 mg/kg) significantly reduced the number of writhing, extended the pain response latency, and suppressed xylene-induced ear swelling. Each EECTD treatment group also had significant inhibition of cotton granulation formation in addition to reduced carrageenan-induced paw edema. EECTD was also shown to alleviate signs of inflammation in histopathological paw sections. However, it had a less noticeable effect on mouse ear swelling in the delayed type hypersensitivity test. A purified compound was isolated from EECTD and its structure was identified as AA. In vitro experimental results showed that both EECTD and AA were able to significantly inhibit the release of pro-inflammatory cytokines NO and PGE2 on LPS-induced RAW264.7 cells. Conclusion These results suggest that EECTD has significant anti-inflammatory and antinociceptive activities, partially related to one of the active substances identified as AA. We hypothesize that these effects are related to its ability to inhibit the production of cytokines NO and PGE2. However, further work will be needed to determine its exact mechanism of action. © 2015 Elsevier Ireland Ltd.

 

通讯机构:College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China
学科代码:肾脏病学 泌尿外科学   关键词:Clematis terniflora DC.提取物抗炎 镇痛 免疫调节活性 乙酸橙酰胺 ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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