自胡桃科青钱柳中提取的两种三萜酸促进3T3-L1脂肪细胞的糖摄取:AMPK激活的相关性

Two triterpeniods from Cyclocarya paliurus (Batal) Iljinsk (Juglandaceae) promote glucose uptake in 3T3-L1 adipocytes: The relationship to AMPK activation
2015-08-27 09:42点击:147次发表评论
作者:Zhu, K.N., Jiang, C.H., Tian, Y.S., Xiao, N., Wu, Z.F., Ma, Y.L., Lin, Z., Fang, S.Z., Shang, X.L., Liu, K., Zhang, J., Liu, B.L. , Yin, Z.Q.
机构: 中国药科大学天然药物化学系 天然药物国家重点实验室
期刊: Phytomedicine2015年7月9期22卷

Purpose The current study investigated the efficacy of Cyclocarya paliurus chloroform extract (CPEC) and its two specific triterpenoids (cyclocaric acid B and cyclocarioside H) on the regulation of glucose disposal and the underlying mechanisms in 3T3-L1 adipocytes. Methods Mice and adipocytes were stimulated by macrophages-derived conditioned medium (Mac-CM) to induce insulin resistance. CPEC was evaluated in mice for its ability by oral glucose tolerance test (OGTT) and insulin tolerance test (ITT). To investigate the hypoglycemic mechanisms of CPEC and its two triterpenoids, glucose uptake, AMP-activated protein kinase (AMPK) activation, inhibitor of NF-κB kinase β (IKKβ) phosphorylation and insulin signaling transduction were detected in 3T3-L1 adipocytes using 2-NBDG uptake assay and Western blot analysis. Results Mac-CM, an inflammatory stimulus which induced the glucose and insulin intolerance, increased phosphorylation of IKKβ, reduced glucose uptake and impaired insulin sensitivity. CPEC and two triterpenoids improved glucose consumption and increased AMPK phosphorylation under basal and inflammatory conditions. Moreover, CPEC and its two triterpenoids not only enhanced glucose uptake in an insulin-independent manner, but also restored insulin-mediated protein kinase B (Akt) phosphorylation by reducing the activation of IKKβ and regulating insulin receptor substrate-1 (IRS-1) serine/tyrosine phosphorylation. These beneficial effects were attenuated by AMPK inhibitor compound C, implying that the effects may be associated with AMPK activation. Conclusions CPEC and its two triterpenoids promoted glucose uptake in the absence of insulin, as well as ameliorated IRS-1/PI3K/Akt pathway by inhibiting inflammation. These effects were related to the regulation of AMPK activity. © 2015 Published by Elsevier GmbH. All rights reserved.

通讯机构:Department of Natural Medicinal Chemistry and State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu Province, China
学科代码:内科学 补充和替代医学 药学   关键词:自胡桃科青钱柳 三萜酸 3T3-L1脂肪细胞 糖摄取 ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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