OPN基因多态性可影响中国人群的膝骨关节炎风险和滑液中OPN水平

Department of Orthopedics, Second Affiliated Hospital of Harbin Medical University, 194 Nangang District, Harbin, 150081, China

Introduction: A body of studies suggests the role of osteopontin (OPN) in onset and development of osteoarthritis (OA), however, the association between OPN polymorphisms and OA susceptibility as well as its clinical features has not been reported.Methods: A total of 750 patients with primary knee OA and 794 healthy volunteer were enrolled as controls. Both OA and control groups were interviewed to obtain demographic and clinical data. Three polymorphisms of OPN gene, namely, -156GG/G, -443C/T and -66T/G were determined. The levels of the full length and the thrombin-cleaved OPN in synovial fluid (SF) from OA subjects were measured.Results: We found the polymorphisms of the -443C/T and the -66/T/G were significantly associated with the OA risk and the radiographic severity. The -443TT and -66GG showed protective effect against developing OA and were associated with lower Kellgren-Lawrence grade. Besides, the polymorphisms of -443C/T and -66T/G significantly affected the thrombin-cleaved OPN levels in SF from OA subjects. Subjects with -443TT and -66GG genotypes had lower thrombin-cleaved OPN levels in SF. The thrombin-cleaved OPN levels in SF were positively correlated to the radiographic severity of OA.Conclusions: Our findings suggest that certain OPN gene polymorphisms may be used as molecular markers for the susceptibility and severity of OA. © 2013 Jiang et al.; licensee BioMed Central Ltd.

Jiang, Y.; Department of Orthopedics, Second Affiliated Hospital of Harbin Medical University, 194 Nangang District, Harbin, 150081, China; email:dryongqingjiang@yahoo.com.cn