BDNF/PI3K/Akt and Nogo-A/RhoA/ROCK信号通路有助于侯氏黑散对大鼠脑缺血性损伤的神经恢复作用

BDNF/PI3K/Akt and Nogo-A/RhoA/ROCK signaling pathways contribute to neurorestorative effect of Houshiheisan against cerebral ischemia injury in rats
2017-03-06 15:20发表评论
作者:Chang, J, Yao, X, Zou, H, Wang, L, Lu, Y, Zhang, Q , Zhao, H.
机构: 首都医科大学中医药学院
期刊: J Ethnopharmacol2016年12月期194卷

Ethnopharmacological relevance Houshiheisan (HSHS), a classic traditional medicine prescription, has notable effects on patients with stroke Aim of the study To investigate the neurorestorative effects of HSHS on ischemic stroke and explore its mode of action. Materials and methods Focal cerebral ischemia models were induced by permanent middle cerebral artery occlusion (pMCAO). Male Sprague-Dawley (SD) rats were randomly divided into 5 experimental groups: sham vehicle, ischemia vehicle, pMCAO+HSHS at 5.1, 10.2 g/kg, and pMCAO+Ginaton 0.028 g/kg. HSHS or Ginaton was administrated 6 h after pMCAO onset. Neurological function was assessed and then rats were sacrificed 7 days after MCAO. Cerebral ischemic injury was evaluated by hematoxylin and eosin (HE) staining and Neuronal nuclear antigen (NeuN) immunofluorescence analysis. The levels of BDNF were detected by enzyme linked immunosorbent assay (ELISA), and the expression levels of PI3K/Akt and Nogo-A/RhoA/ROCK2 signaling pathway were detected by western blot and quantitative real-time PCR (qRT-PCR). Results Compared with those results of pMCAO group, HSHS 5.1 and HSHS 10.2 groups markedly improved neurological function, alleviated pathological damage, promoted the neuronal survival, increased the expression of BDNF, PI3K, Akt, in protein and mRNA, decreased the expression of Nogo-A, NgR, RhoA and ROCK2 in protein and mRNA 7 days after pMCAO. Conclusions The findings demonstrate that HSHS had significant therapeutic effects on ischemic stroke and it perhaps worked through the activation of BDNF/PI3K/Akt and down-regulation of Nogo-A/RhoA/ROCK signaling pathways. © 2016 Elsevier Ireland Ltd

通讯机构:School of Traditional Chinese Medicine, Capital Medical University, Beijing, China
学科代码:内科学 补充和替代医学 药学   关键词:侯氏黑散 脑缺血 神经 ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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